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KMID : 0525720080130040225
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Journal of Chitin and Chitosan
2008 Volume.13 No. 4 p.225 ~ p.229
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Chemical Modification of Chitosan with 1-Aziridineethanol for the Increase of Amine Values
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Jung Hyun
Jang Mi-Kyeong
Nah Jae-Woon
Kim Yang-Bae
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Abstract
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To imporve transfection efficiency, we prepared 1-aziridineethanol-low molecular weight water-soluble chitosan(LMWSC) having the potential to form complex with DNA as a cationic polymer. 1-aziridineethanol-LMWSC(LMWSC-EA) was synthesized by ring opening reaction of 1-aziridineethanol with chitosan. The LMWSC-EA was characterized using H-NMR×? 13 C-NMR. LMWSC-EA was complexed with plasmid DNA(pDNA) in various polymer/DNA(N/P) weight ratios, and the complex was identified using gel retardation assay. Therefore, LMWSC-EA will be useful in the development of gene carriers.
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KEYWORD
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LMWSC, 1-Aziridineethanol, transfection efficiency
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